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Additional info for BSAVA Guide to Procedures in Small Animal Practice (BSAVA British Small Animal Veterinary Association)
6. Examine the samples for haemolysis (reddening of the supernatant). 7. Examine and score the tubes for agglutination. A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 40 A B C D E F G H I J K Procedures in Small Animal Practice 8. This should not be confused with rouleaux formation. 9. For the recipient control: a. If there is no haemolysis or agglutination in the recipient control tube, the results are valid and incompatibilities can be interpreted b. If there is haemolysis or agglutination present in the recipient control tube, then the compatibility and suitability of the donor cannot be accurately assessed.
4. Record the values for 3–5 cycles and report the averages for systolic, diastolic and mean pressures. L Potential false readings M Incorrect blood pressure readings may be obtained due to: • Inappropriate cuff size • Inappropriate placement of the cuff • Excessive motion of the limb or tail • Low blood pressure • Dysrhythmias • Obesity • Peripheral oedema • Limb conformation, which does not permit snug placement of the cuff • Stress. 5 ml of heparin sodium is aspirated into the 2 ml syringe via the needle and then expelled.
Major cross-match = donor red blood cells and recipient plasma b. Minor cross-match = recipient red blood cells and donor plasma c. Recipient control = recipient red blood cells and recipient plasma. 3. Gently rock the slides to mix the plasma and red blood cells. Examine for agglutination after 1–5 minutes. 4. For the recipient control: agglutination will invalidate results. S Results of cross-matching L M N O P Q T U V W X Y Z • Any agglutination and/or haemolysis is a ‘positive’ result. This makes interpretation of the test diffi cult, although it can be repeated with additional washing of the recipient’s red blood cells.